I am particular about the thread I use for hand applique. I always use 100% cotton thread when sewing cotton fabric. I recommend Masterpiece by Superior for my hand applique.
I like to work from thread wound onto bobbins because they are small and lightweight, making it easy to carry many colors with you. We worked with the fine people at Superior to choose the 35 colors of thread in this bobbin set. Each bobbin holds roughly 85 yards of 2-ply, 100% cotton Masterpiece thread.
Please note that Masterpiece on the larger spools and cones is a 3-ply thread, too thick for hand applique. For applique, use the 2-ply Masterpiece that comes only on bobbins.